Inverse Single-Strand RACE: An Adapter-Independent Method of 5′RACE
نویسندگان
چکیده
منابع مشابه
Inverse single-strand RACE: an adapter-independent method of 5' RACE.
With the advent of techniques for gene isolation based on polymerase chain reaction (PCR), there is an increasing need for methods to complete missing cDNA parts, especially in the more difficult 5′ side. While library construction and screening is always a possibility, the process is time-consuming and does not promise isolation of complete cDNAs. For this reason, several PCR-based methods hav...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99274bm04